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Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
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Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
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Bio-Rad cfx 96tm real time pcr detection system
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
Cfx 96tm Real Time Pcr Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad bio rad cfx manager 3 0 software
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
Bio Rad Cfx Manager 3 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad manager 3 0 software
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
Manager 3 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad cfx manager3 0 software
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
Cfx Manager3 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad bio rad cfx manager 3 0 system
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
Bio Rad Cfx Manager 3 0 System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad bio rad cfx96 real time detection system
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
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Bio-Rad bio plex pro diabetes assay
Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 <t>ProteinChip</t> arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.
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Image Search Results


Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 ProteinChip arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.

Journal: FEBS Open Bio

Article Title: Proteomic pattern of breast milk discriminates obese mothers with infants of delayed weight gain from normal‐weight mothers with infants of normal weight gain

doi: 10.1002/2211-5463.12610

Figure Lengend Snippet: Heat map/hierarchical clustering of 15 breast milk proteins of 52 mothers divided into two groups, normal weight ( n = 26) and obese ( n = 26). The clusters are obtained by combining the average intensity values of the samples tested in duplicate on CM 10 and Q10 ProteinChip arrays using the following acquisition protocol: laser energy, 3500 nJ; focus mass, 10 000 Da; matrix attenuation, 3000 Da. Above the heat map are shown the sample names (numbered from 1 to 26 in each group, in red for the mothers of normal weight (normoponderal), and in blue for the obese mothers). On the right side of the image are the molecular masses detected on ProteinChip arrays of CM 10 (red) and Q10 (blue) type. EDM conditions: first pass: peak S/N, ≥ 5; valley depth S/N, ≥ 2; minimal peak threshold, 20% of all spectra; second pass: peak S/N, ≥ 2; valley depth S/N, ≥ 2; third pass: adding estimated (missing) peaks to complete clusters; clustered mass window width, 0.1%; m / z range of analysis ( z = 1), 3000–30 000 Da.

Article Snippet: For spectra processing, expression difference mapping (EDM) was carried out using proteinchip data manager 3.0.7 software (Bio‐Rad).

Techniques: